PCR Device Development

Do you need help developing a PCR device for your molecular diagnostic testing device?

Commercializing Your Proprietary PCR Device 

Transitioning your PCR development from the laboratory to a commercialized product has many challenges.  At Symbient, a Gener8 company, we utilize our engineering expertise and scientific understanding to help our clients develop a PCR testing device that is ready for use in the market. Here are just some of the ways our PCR device development can work to solve many of the most common PCR challenges:

Reagent Storage

Challenge

The commercialization of reagent storage can have challenges with shelf life, travel, and/or stability at room temperature.

Solution

We are well versed in the integration of liquid reagents, lyophilized beads, and dry-down reagents.  We have designed for blister packs, custom foil sealed components, and other low moisture barrier solutions in cartridges.  

Lysing Cell

Challenge

Depending on what you are testing for, the vitality of certain cells can range from resilient to delicate, therefore your technology requires a balance in order to process a wide range of cells without inhibiting results.

Solution

Cell Lysis can be achieved mechanically or passively. With our experience and prototyping capabilities, a commercial solution can quickly be determined.

Sample Prep

Challenge

Isolating or capturing your complete sample is critical to result in enough sample for successful detection.  

Solution

We have employed many solutions for magnetic bead separation and capture as well as filtering to isolate samples.

Master Mix

Challenge

Combining a Master mix with a sample can be easy in the laboratory but mixing on a commercialized product poses it’s challenges.  

Solution

We have integrated many mixing techniques that are built into the cartridge geometry and/or are driven by on-instrument components.

 Thermocycling (Heating)

Challenge

For PCR to be successful, temperatures need to rise and fall quick enough so that the cells may grow and multiply.   

Solution

We can design a cartridge with either single sided or dual sided exposure to the Thermo Electric Cooling (TEC).  Our cartridge and instrument teams work together to identify the best solution to meet your requirements. 

Bubbles

Challenge

Thermocycling often creates bubbles which can interfere with detection. These need to be removed for testing and commercialization of the cartridge.

Solution

A chemical additive may not be a desired option. In these cases, we have been successful using filters or the cartridge architecture to capture bubbles.

For more information on our PCR device development and capabilities, get in touch with us or continue below to find some case studies on PCR devices we’ve produced in the past:

PCR Cartridge
PCR Cartridge 2